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KMID : 0364820130490020112
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Korean Journal of Microbiology
2013 Volume.49 No. 2 p.112 ~ p.117
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Development of PCR Diagnosis System for Plant Quarantine Seed-borne Wheat Streak Mosaic Virus
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Lee Si-Won
Kang Eun-Ha
Chu Yeon-Mee
Shin Young-Gil
Ahn Tae-Young
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Abstract
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Wheat streak mosaic virus (WSMV), a member of the genus Tritimovirus in Potyviridae, severely impacts wheat and
corn seed worldwide, but has yet to be detected in Korea, and hence, every effort should be made to prevent its introduction. To prevent WSMV from entering the country, it is necessary to prepare a specific, sensitive, simple, and fast detection method for routine application to plant quarantine procedures. For this reason, a two-step
diagnosis system consisting of RT-PCR and nested PCR is being used for WSMV detection. In addition, a novel positive control was developed for use with the system. WSMV has been detected in seed sweet corn from Japan and seed wheat from USA by a two-step diagnosis system, the details of which are described in this study. After sequence analysis, similarities of 80.6 and 100.0% with other isolates were determined by BLAST. They showed the same topology, which was classified as 4 genotypes by various phylogenetic trees, using a poly protein encoding sequence amplification. In this analysis, WSMV-JSweet-corn2868 (JX845574) is classified as clade B, while WSMV-Uwheat1944-1 (KC754959) and WSMV-Uwheat1944-2 (KC754960) belong to clade D.
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KEYWORD
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quarantine, RT-PCR, seed-borne virus, WSMV
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